For research use only.
| Verified Samples | Verified Samples in WB: Mouse kidney Verified Samples in IHC: Human liver cancer, Human tonsil |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human MEST |
| Abbre | MEST |
| Synonyms | DKFZp686L18234, EC 3.-.-.-, MEST, MGC111102, MGC8703, Mesoderm specific transcript (mouse) homolog, Mesoderm specific transcript homolog (mouse), Mesoderm-specific transcript homolog protein, OTTHUMP00000210362, OTTHUMP00000210364, OTTHUMP00000210367, Paternall |
| Swissprot | |
| Calculated MW | 39 kDa |
| Observed MW | Refer to figures The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endoplasmic reticulum membrane. |
| Concentration | 0.9 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Cell Biology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | MEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene,which is associated with growth of mesodermal origin cells and plays important roles in embryo development. It is also a novel regulator of Wnt/CTNNB signalling during adipogenic differentiation. It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site. |
Other Clones
1 Results
Other Formats
1 Results
Unconjugated
