Malondialdehyde (MDA) Fluorometric Assay Kit (E-BC-F007)
For research use only.
| Detection Principle | MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which is proportional to the fluorescence intensity at the excitation wavelength of 520 nm and emission wavelength of 550 nm. |
| Synonyms | MDA |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=520 nm/550 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Glacial acetic acid, Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.04 μmol/L |
| Detection Range | 0.04-10 μmol/L |
| Precision | inter-assay CV: 4.5-7.0% | intra-assay CV: 2.3-4.3% |
| Sample Volume | 100 μL |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 2-4 |
| Human plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Mouse serum | 2-4 |
| Mouse plasma | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 2-4 |
| 9.2 × 10^6 CHO cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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