Low-Density Lipoprotein Cholesterol (LDL-C) Colorimetric Assay Kit (Double Reagents) (E-BC-K205-S)
For research use only.
| Detection Principle | Lipoproteins (except low-density lipoprotein cholesterol (LDL)) such as high density lipoprotein (HDL), chylomicron (CM), and very low density lipoprotein (VLDL) change structure and dissociate under the action of surfactants. The released micronized cholesterol molecules react with cholesterol enzyme reagents, and the generated hydrogen peroxide is trapped in the absence of coupling agent. It is consumed without color development. At this time, the LDL particles are still intact, and then the reagent containing coupling agent is added, which can dissociate the LDL particles to release cholesterol, which is catalyzed by cholesterol esterase (CE) and cholesterol oxidase (CO) and produce hydrogen peroxide. Hydrogen peroxide is catalyzed by oxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound, the shade of color is directly proportional to the LDL-C content. |
| Sample Type | Serum,Plasma,animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (546 nm) |
| Research Area | Metabolic Diseases |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Isopropyl alcohol (AR) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.061 mmol/L |
| Detection Range | 0.061-12 mmol/L |
| Precision | inter-assay CV: 3.2-7.3% | intra-assay CV: 1.1-4.8% |
| Sample Volume | 20μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Porcine serum | 1 |
| Rabbit serum | 1 |
| Horse serum | 1 |
| 10% Mouse lung tissue homogenization | 1 |
| 10% Mouse kidney tissue homogenization | 1 |
| 10% Mouse liver tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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