Lipoxygenase (LOX) Activity Assay Kit (E-BC-K861-M)
For research use only.
| Detection Principle |
Lipoxygenase（LOX）is widely found in plant tissues, especially in seeds with high oil content such as soybean. LOX catalyzes the oxidation of unsaturated fatty acids, leading to membrane lipid peroxidation. It plays an important role in plant growth and development, maturation and senescence and stress. LOX can catalyze linoleic acid oxidation, the oxidation product has the specific absorption peak at 280 nm. The activity of LOX can be calculated by measuring the change of absorbance value at 280 nm. |
| Synonyms | LOX |
| Sample Type | Plant sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (270-290 nm, optimum wavelength: 280 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | Double distilled water |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 5 U/L |
| Detection Range | 5-339.5 U/L |
| Precision | inter-assay CV: 0.05 | intra-assay CV: 0.04 |
| Sample Volume | 20 μL(tissue homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Long bean | 1 |
| 10% Green soy bean | 2-5 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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