Lgals3 Polyclonal Antibody (E-AB-40655)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela, MCF-7, A431, RAW264.7, NIH/3T3, C6 |
| Dilution | WB 1:250-1:500 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Rat Lgals3 protein expressed by E.coli |
| Abbre | Lgals3 |
| Synonyms | 35 kDa lectin, CBP 35, GALBP, Gal-3, Galactose-specific lectin 3, Galectin-3, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, MAC2, Mac-2 antigen, LGALS3 |
| Swissprot | |
| Calculated MW | 26 kDa |
| Observed MW |
28 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Neuroscience, Cell Biology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes. |
Other Clones
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Other Formats
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Unconjugated
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