LAMP2/CD107b Monoclonal Antibody (AN200230P)
For research use only.
| Verified Samples |
Verified Samples in WB:?HepG2, Jurkat, Hela Verified Samples in IF: Hela |
| Dilution | WB 1:500-1:2000, ICC/IF 1:20-1:100 |
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB, ICC/IF |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human LAMP2/CD107b Protein |
| Abbre | LAMP2 |
| Synonyms | Lysosome-Associated Membrane Protein, Lysosome-Associated Membrane Glycoprotein, LGP, LAMP2, CD107b, LAMP-2, LAMPB, LGP-96, LGP110, CD107 Antigen-Like Family Member B, Lysosome-Associated Membrane Glycoprotein 2, Lysosome-Associated Membrane Protein 2, CD107 antigen like family member B, LAMP 2, LAMP 2C, LGP 96, LGP96, Lysosomal associated membrane protein 2, Lysosome associated membrane glycoprotein 2, Lysosome associated membrane protein 2, MAC3 |
| Swissprot | |
| Calculated MW | 45 kDa |
| Observed MW |
100-120 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Tags & Cell Markers, Signal Transduction, Immunology, Cardiovascular, Stem Cells, Metabolism, Cancer |
| Clone No. | 5B11 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. |
Other Clones
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Other Formats
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Unconjugated
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