Lactase Activity Assay Kit (E-BC-K131-M)

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Detection Principle	Lactase decomposes lactose to produce glucose. Under the action of enzyme, glucose produces hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide to produce colored substances. Lactase activity can be calculated by measuring the OD value at 505 nm.
Sample Type	animal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (495-510 nm, optimum wavelength: 505 nm)
Research Area	Glycolysis And Lipid Metabolism
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	3.94 U/mL
Detection Range	12.5-2000 U/mL.
Precision	inter-assay CV: 8.5% \| intra-assay CV: 4.5%
Sample Volume	50 μL
Assay Time	1 h

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Rat lleal tissue homogenate	1
10% Rat jejunum tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat kidney tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.