L-Selectin/CD62L Polyclonal Antibody(Capture/Detector) (AN003950P)
For research use only.
| Verified Samples |
Verified Samples in ELISA: Recombinant Mouse L-Selectin/CD62L protein, Mouse serum, Mouse plasma Verified Samples in WB: Mouse serum |
| Dilution | ELISA Capture 2-8 μg/mL, ELISA Detector 0.1-0.4 μg/mL, WB 1:500-1:1000 |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Mouse |
| Applications | ELISA Capture/Detector, WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Mouse L-Selectin/CD62L Protein expressed by Mammalian |
| Abbre | L-Selectin/CD62L |
| Synonyms | Lymphocyte antigen, Leukocyte adhesion molecule, Ly-m, CD62 antigen-like family member L, CD62L, LAM1, LAM-1, LECAM1, LECAM-1, LEU8, Leu-8, Leukocyte adhesion molecule 1, LSEL, L-selectin, Lyam1, Lyam-1, Ly-m22, Lymph node homing receptor, Lymphocyte antigen 22, PLNHR, TQ1, Lnhr, Ly-22, Ly22, Sell, LECAM-1, LAM-1, Ly-22, MEL-14 |
| Swissprot | |
| Calculated MW | 42 kDa |
| Observed MW |
80-100 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300. |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. |
Other Clones
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Unconjugated
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