L-Lactic Acid (LA) Colorimetric Assay Kit (E-BC-K044-S)
For research use only.
| Detection Principle | Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm. |
| Sample Type | Serum,Plasma,Cell culture supernatant,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (530 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.05 mmol/L |
| Detection Range | 0.05-6.0 mmol/L |
| Precision | inter-assay CV: 1.9% | intra-assay CV: 1.1% |
| Sample Volume | 20 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 4-8 |
| Rat serum | 4-8 |
| 10% Mouse muscle tissue homogenization | 2-4 |
| 10% Mouse liver tissue homogenization | 1 |
| HePG2 cells homogenization (1.388 gprot/L) | 4-8 |
| HepG2 supernatant | 2-4 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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