KRCC1 Polyclonal Antibody (E-AB-19203)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse brain, Mouse kidney Verified Samples in IHC: Human tonsil |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human KRCC1 |
| Abbre | KRCC1 |
| Synonyms | CHBP2, Cryptogenic hepatitis-binding protein 2, KRCC1, Lysine-rich coiled-coil protein 1, cryptogenic hepatitis binding protein, lysine rich coiled coil 1 |
| Swissprot | |
| Calculated MW | 31 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1.68 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cell Biology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | KRCC1 (lysine-rich coiled-coil 1), also known as CHBP2 (cryptogenic hepatitis-binding protein 2), is a 259 amino acid protein that is encoded by a gene located on human chromosome 2p11.2.Consisting of 237 million bases, chromosome 2 is the second largest human chromosome and encodes over 1,400 genes.A number of genetic diseases are linked to genes on chromosome 2.Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the ABCA12 gene.The lipid metabolic disorder sitosterolemia is associated with ABCG5 and ABCG8.An extremely rare recessive genetic disorder, Alstr m syndrome, is due to mutations in the ALMS1 gene.Interestingly, chromosome 2 contains what appears to be a vestigial second centromere and vestigial telomeres which gives credence to the hypothesis that human chromosome 2 is the result of an ancient fusion of two ancestral chromosomes seen in modern form today in apes. |
Other Clones
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Unconjugated
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