KCNK15 Polyclonal Antibody (E-AB-18167)

For research use only.
Verified Samples |
Verified Samples in WB: Human muscle Verified Samples in IHC: Human tonsil |
Dilution | WB 1:1000-1:5000, IHC 1:25-1:100 |
Isotype | IgG |
Host | Rabbit |
Reactivity | Human |
Applications | WB, IHC |
Clonality | Polyclonal |
Immunogen | Synthetic peptide of human KCNK15 |
Abbre | KCNK15 |
Synonyms | Acid sensitive potassium channel protein TASK 5, Acid-sensitive potassium channel protein TASK-5, K2p15.1, KCNK11, KCNK14, KCNKF, KIAA0237, KT3.3, Kcnk15, OTTHUMP00000031072, Potassium channel subfamily K member 14, Potassium channel subfamily K member 15, dJ781B1.1 |
Swissprot | |
Calculated MW | 36 kDa |
Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Membrane. |
Concentration | 1.26 mg/mL |
Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
Purification Method | Antigen affinity purification |
Research Areas | Neuroscience |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
background | This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel, however, it may require other non-pore-forming proteins for activity. KCNK15 (Potassium Two Pore Domain Channel Subfamily K Member 15) is a Protein Coding gene. Among its related pathways are Neuropathic Pain-Signaling in Dorsal Horn Neurons and Sweet Taste Signaling. GO annotations related to this gene include potassium channel activity and potassium ion leak channel activity. An important paralog of this gene is KCNK9. |
Other Clones
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Other Formats
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Unconjugated
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