Intracellular Acidification Rate Fluorometric Assay Kit (E-BC-F073)
For research use only.
| Detection Principle |
Intracellular acidification rate refers to the degree of change in the pH of the intracellular environment, which is an important parameter for the study of cell function. Constant intracellular pH level is an indispensable condition for maintaining normal cell metabolism and function. Most physiological and biochemical reactions in the cell, such as cell growth, calcium regulation, enzyme activity, receptor mediated signal transduction, ionic action, endocytosis, and cell adhesion, etc. Therefore, there is a close relationship between intracellular pH regulation and metabolism, and accurate detection of intracellular pH fluctuations is an important way to understand intracellular metabolism. At the same time, studying the interaction between intracellular pH regulation and metabolism will help to better understand the biochemical processes in cells and provide new ideas and methods for the treatment of related diseases. The probe used in this test box has the characteristics of dual excitation wavelength. The probe has the maximum absorption at 490 nm and an equal absorption point at 440 nm. By calculating the ratio of the two excitation wavelengths, the fluctuation of intracellular pH can be detected. |
| Sample Type | Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex=490 nm/440 nm; Em=535 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Assay Time | 1 h |
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® Violet 450
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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