ILKAP Polyclonal Antibody (E-AB-52624)
For research use only.
| Verified Samples |
Verified Samples in WB: 293T Verified Samples in IHC: Human ovarian cancer |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human ILKAP |
| Abbre | ILKAP |
| Synonyms | ILKAP, ILKAP2, ILKAP3, PP2C-DELTA, PPM1O, DKFZp434J2031, FLJ10181, Integrin linked kinase associated phosphatase, Integrin linked kinase associated serine/threonine phosphatase, Integrin linked kinase associated serine/threonine phosphatase 2C, Integrin-linked kinase-associated serine/threonine phosphatase 2C, MGC4846, PP2C DELTA, Protein phosphatase 2c delta isozyme |
| Swissprot | |
| Calculated MW | 43 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm. |
| Concentration | 0.96 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway.ILKAP (ILK Associated Serine/Threonine Phosphatase) is a Protein Coding gene. Among its related pathways are AKT Signaling Pathway. GO annotations related to this gene include protein serine/threonine phosphatase activity. An important paralog of this gene is PPM1F. |
Other Clones
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Unconjugated
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