IL7R Polyclonal Antibody (E-AB-17919)
For research use only.
| Verified Samples |
Verified Samples in WB: Human fetal brain, Jurkat Verified Samples in IHC: Human thyroid cancer |
| Dilution | WB 1:500-1:2000, IHC 1:40-1:200 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human IL7R |
| Abbre | IL7R |
| Synonyms | CDW, IL7R, CD127, CDW127, IL-7R-alpha, IL7RA, ILRA, IL-7R, IL-7RA, IL-7Rα, Interleukin-7 receptor subunit alpha, IL-7R&, alpha, CD 127, CD127 antigen, IL 7R, IL 7R alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL7Ralpha, Interleukin 7 receptor, Interleukin 7 receptor alpha chain, Interleukin 7 receptor isoform H5 6 |
| Swissprot | |
| Calculated MW | 52 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted and Cell membrane. |
| Concentration | 1.32 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Immunology, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found. |
Other Clones
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Other Formats
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Unconjugated
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