IL12A Polyclonal Antibody (E-AB-92232)
For research use only.
| Verified Samples |
Verified Samples in WB: RAW264.7, Recombinant IL12A/NKSF1 Protein |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | A synthetic peptide of human IL12A |
| Abbre | IL12A |
| Synonyms | NKSF, IL-12/IL-35 p, IL-12 subunit p, Interleukin 12/P, NK cell stimulatory factor chain, CLMF p, P35, CLMF, NFSK, NKSF1, IL-12A, CLMF p35, CLMF1, Cytotoxic Lymphocyte Maturation Factor 35 kDa, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12/IL-35 p35, Interleukin-12 subunit alpha, NK cell stimulatory factor chain 1, IL12A, IL12B, Interleukin 12/P70, CLMF, IL-12A, NFSK, NKSF1 |
| Swissprot | |
| Calculated MW | 24 kDa |
| Observed MW |
35 kDa/75 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endoplasmic reticulum lumen, extracellular region, extracellular space, interleukin-12 complex, late endosome lumen. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Immunology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. |
Other Clones
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Other Formats
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Unconjugated
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