IL-32 alpha/IL32A Monoclonal Antibody (AN200201P)
For research use only.
| Verified Samples | Verified Samples in WB:?Jurkat |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG2a |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human IL-32 alpha/IL32A Protein |
| Abbre | IL32 |
| Synonyms | TAIFa, IL-32alpha, IL-32beta, IL-32delta, IL-32gamma, TAIFb, TAIFc, TAIFd, IL32, NK4, TAIF, IL 32alpha, IL 32beta, IL 32delta, IL 32gamma, IL-32, Interleukin 32, Interleukin 32 small, Interleukin 32 theta, Interleukin-32, interleukin-32 eta, Natural killer cell transcript 4, Natural killer cells protein 4, NK 4, OTTHUMP00000236040, OTTHUMP00000236041, OTTHUMP00000236042, OTTHUMP00000236043, OTTHUMP00000236044, OTTHUMP00000236045, OTTHUMP00000236046, OTTHUMP00000236047, OTTHUMP00000236048, OTTHUMP00000236049, OTTHUMP00000236050, OTTHUMP00000236051, OTTHUMP00000236052, OTTHUMP00000236053, OTTHUMP00000236054, OTTHUMP00000241545, OTTHUMP00000241602, OTTHUMP00000241603, OTTHUMP00000241604, OTTHUMP00000241605, OTTHUMP00000241606, OTTHUMP00000241607, OTTHUMP00000241608, OTTHUMP00000241609, OTTHUMP00000241610, OTTHUMP00000241611, OTTHUMP00000241612, Tumor necrosis factor alpha-inducing factor |
| Swissprot | |
| Calculated MW | 26 kDa |
| Observed MW |
30 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Selectively expressed in lymphocytes. Expression is more prominent in immune cells than in non-immune cells. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Cancer |
| Clone No. | 6H3 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma, and delta) are the most representative IL-32 transcripts, and the gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, Mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis, and in the human stomach cancer, human lung cancer, and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer. |
Other Clones
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Unconjugated
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