IL-1 beta Monoclonal Antibody (AN200016P)
For research use only.
| Verified Samples | Verified Samples in WB: THP-1 |
| Dilution | WB 1:500-1:2000, |
| Isotype | IgG2b |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | A synthetic peptide corresponding to the center region of the Human IL-1 beta |
| Abbre | IL1B |
| Synonyms | IL1F, IL-1F, pro IL1F, IL-1, IL1-BETA, IL1F2, IL1 beta, IL1B, catabolin, IL-1B, pro Catabolin, pro IL1B, pro IL1F2, pro leukocytic endogenous mediator, pro leukocytic pyrogen, pro lymphocyte activating factor, pro mononuclear cell factor, IL-1F2, IL 1B, IL-1 beta |
| Swissprot | |
| Calculated MW | 31 kDa |
| Observed MW |
18 kDa, 34 kDa, 36 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Cytosol, Secreted, Lysosome, Secreted, Extracellular exosome. |
| Tissue Specificity | Monocytes/macrophages. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Cardiovascular, Metabolism |
| Clone No. | 7F13 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. |
Other Clones
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Unconjugated
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