Hydrogen Sulfide (H2S) Colorimetric Assay Kit (Indirect Method) (E-BC-K355-M)
For research use only.
| Detection Principle | H2S can react with acetate solution to form ZnS which can be dissolved in alkaline solution. In the presence of Fe3+, methylene blue can be formed. Methylene blue has a maximum absorption peak at 665 nm. H2S content can be calculated indirectly by measuring the OD value at 665 nm. |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (660-670 nm, optimum wavelength: 665 nm) |
| Research Area | Other |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.15 μmol/L |
| Detection Range | 0.76-100 μmol/L |
| Precision | inter-assay CV: 9.9% | intra-assay CV: 3.1% |
| Sample Volume | 100 μL |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human plasma | 1 |
| Mouse plasma | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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