Human HSP-27/HSPB1 (Heat Shock Protein 27) ELISA Kit

Size	Price	Qty
96T	$ 495.00
48T	$ 396.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.15-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reacitivity	Human
Specificity	This kit recognizes HSP-27 in samples.No significant cross-reactivity or interference between HSP-27 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HSP-27 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HSP-27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HSP-27 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of HSP-27 . You can calculate the concentration of HSP-27 in the samples by comparing the OD of the samples to the standard curve.

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.

Uniport ID	P04792
Research Area	Cancer , Cardiovascular , Signal Transduction

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Human HSP-27/HSPB1 (Heat Shock Protein 27) ELISA Kit (E-EL-H6290)

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