Human CCL16(Chemokine C-C-Motif Ligand 16) ELISA Kit (E-EL-H0701)

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Size	Price	Qty
96T	$ 609.00
48T	$ 487.00
24T	$ 150.00
96T*5	Inquire	/

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Product Summary

Sensitivity	0.1 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reacitivity	Human
Specificity	This kit recognizes Human CCL16 in samples. No significant cross-reactivity or interference between Human CCL16 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T*5
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CCL16. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CCL16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CCL16, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CCL16. You can calculate the concentration of Human CCL16 in the samples by comparing the OD of the samples to the standard curve.

Background

CCL16 Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.

Gene Alias	CCL16
Gene ID	6360
Uniport ID	O15467
Protein Alias	CCL16
Research Area	Immunology

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