Human ACTH(Adrenocorticotropic Hormone) ELISA Kit (E-EL-H0137)
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For research use only.
Sensitivity | 9.38 pg/mL |
Detection Range | 15.63-1000 pg/mL |
Sample Volume | 50 μL |
Total Assay Time | 2 h 30 min |
Reacitivity | Human |
Specificity | This kit recognizes Human ACTH in samples.No significant cross-reactivity or interference between Human ACTH and analogues was observed |
Recovery | 80%-120% |
Sample Type | Serum, plasma and other biological fluids |
Detection Method | Colorimetric method, ELISA, Competitive |
Assay Type | Competitive-ELISA |
Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
Storage | 2-8℃ |
Expiration Date | 12 months |
Uniport ID | P01189 |
Research Area | Cancer, Metabolism, Neuroscience, Signal Transduction |
Other Clones
1 Results
Other Formats
1 Results
- Lacticaseibacillus paracasei 207-27 alters the microbiota–gut–brain axis to improve wearable device-measured sleep duration in healthy adults: a randomized, double-blind, placebo-controlled trial
IF:5.1
Journal:Food & Function(2024)
DOI:10.1039/D4FO01684JReactivity:Human
Sample Type:Serum
- The Effect of Nonstress Device Noise Level on Stress Parameters in Primigravid Women: A Randomized Controlled Trial
IF:2.7
Journal:JOURNAL OF MIDWIFERY & WOMENS HEALTH(2024)
DOI:10.1111/jmwh.13581Reactivity:Human
Sample Type:Saliva Samples
- Psychological and Biological Stress Pathways as Common Mechanisms Underlying a Psycho-Neurological Symptom Cluster in Cancer Patients: Perceived Stress, Cortisol, and ACTH
IF:2.7
Journal:European Journal of Oncology Nursing(2024)
DOI:10.1016/j.ejon.2024.102728Reactivity:Human
Sample Type:serum
- The effect of pectointercostal fascial block on stress response in open heart surgery
IF:1.2
Journal:Saudi Journal of Anaesthesia(2024)
DOI:10.4103/sja.sja_349_23Reactivity:Human
Sample Type:blood
- Hypothalamus-pituitary-adrenal axis in patients with post-traumatic stress disorders and related to oxidative stress
IF:1.1
Journal:Hormone Molecular Biology and Clinical Investigation(2024)
DOI:10.1515/hmbci-2024-0017Reactivity:Human
Sample Type:serum
- Stress and psoriasis: Exploring the link through the prism of hypothalamo-pituitary-adrenal axis and inflammation
IF:4.700
Journal:JOURNAL OF PSYCHOSOMATIC RESEARCH(2023)
DOI:10.1016/j.jpsychores.2023.111350Reactivity:Human
Sample Type:serum
- The relationship between liver function and neurophysiological factors in depressed individuals: a cross-sectional study using an integrated “East meets West” medicine approach
IF:4.700
Journal:Frontiers in Psychiatry(2023)
DOI:10.3389/fpsyt.2023.1159785Reactivity:Human
Sample Type:plasma
- Effect of maternal cortisol levels on fetal heart rate patterns in primiparous pregnant women in the third trimester
IF:1.400
Journal:Revista da Associacao Medica Brasileira(2023)
DOI:10.1590/1806-9282.20221610Reactivity:Human
Sample Type:saliva
- Neuroendocrine stress hormones associated with short-term exposure to nitrogen dioxide and fine particulate matter in individuals with and without chronic obstructive pulmonary disease: A panel study in Beijing, China
IF:9.988
Journal:ENVIRONMENTAL POLLUTION(2022)
DOI:10.1016/j.envpol.2022.119822Reactivity:Human
Sample Type:serum
- Differential Health Effects on Inflammatory, Immunological and Stress Parameters in Professional Soccer Players and Sedentary Individuals after Consuming a Synbiotic. A Triple-Blinded, Randomized, Placebo-Controlled Pilot Study
IF:5.719
Journal:Nutrients(2021)
DOI:10.3390/nu13041321Reactivity:Human
Sample Type:Whole Blood
Q1:Why is it necessary to add a protease inhibitor in tissue sample preparation during an Elisa experiment? Will it affect the detection significantly if there is no protease inhibitor?
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
Q2:Which variant of the spike protein does this kit detect? Do you have a kit specific to detect the spike protein for the omicron variant?
This kit is designed for the original strain of the new crown virus, and the omicron variant has not been verified. However, we have verified 26 recombinant variants of the SARS-CoV-2 spike protein through the kit. For more information, customers can refer to the kit instructions (https://file.elabscience.com/Manual/covid_19/E-EL-E605 .pdf).
Q3:What is the range of enzyme activity of your IL-2 freeze-dried powder
Currently our freeze-dried powder is a concentration unit with no information on the activity unit for the time being.
Q4:What is the principle of adding stop solution to stop color reaction in ELISA experiment?
On the one hand, the activity of HRP enzyme is destroyed and the catalytic function of HRP enzyme is lost. On the other hand, the final color changes due to a change in pH.
Q5:The absorbance of the cell supernatant is less than that of the sample diluent alone, so how to concentrate the sample?
The amount of medium can be reduced for subsequent drug administration and modeling.
Q6:My sample volume is small. Can I reduce some reagents proportionally?
No, the detection system of our kits requires strict adherence to the specified sample volume to ensure accurate detection. If the sample volume is insufficient, consider diluting appropriately, but first conduct a pre-experiment to confirm the suitable dilution factor.
Q7:May I ask which type of plate to choose when ELISA testing?
According to the different bottom, it is divided into flat bottom, U-shaped bottom, V-shaped bottom and so on. The index of refraction of the flat bottom is low, which is suitable for detection in the enzyme reader. According to the color can be divided into transparent, black, white. Transparent is commonly used for the most general enzyme-linked immunoassay.
Q8:Is the TGF-β1 ELISA Kit detecting the active form of TGF-β1 or the precursor?
The active TGF-β dimer was detected
Q9:I need to measure corticosterone and testosterone in hair samples. Are there any suggested sample extraction methods?
Hair sample preparation method: Wash hair samples with methanol by adding 5 mL of HPLC-grade methanol to each sample, rotating for 3 minutes, then decanting excess methanol and rinsing hair twice. After washing, place the hair samples on aluminum foil, dry for 3 days in a protective cap. Weigh the dried hair samples and transfer them to 2ml polypropylene tubes containing stainless steel grinding beads. Place the tubes containing hair and beads in a bead beater, grind each sample for 2 minutes to produce powder. After grinding, add 1.5 mL of methanol to the tubes containing hair powder. Rotate samples slowly for 24 hours to extract steroids. Centrifuge at 10000 g for 4 minutes, transfer 0.6 mL of methanol supernatant containing steroids to new 1.5 mL microcentrifuge tubes. Dry the samples in a protective hood for 2-3 days to evaporate the methanol. Dilute the dried extract with 0.4 mL dilution buffer from the kit for detection.
Q10:I got nothing on the IL-18 standard
The standard product is placed in the reagent bottle and then freeze-dried. You can first centrifuge the reagent bottle with 10000×g for 1min, and then directly observe the bottom or side wall of the reagent bottle.
