HNRNPH2 Polyclonal Antibody (E-AB-52276)
For research use only.
| Verified Samples |
Verified Samples in WB: Human liver Verified Samples in IHC: Human esophagus cancer, Human prostate cancer |
| Dilution | WB 1:500-1:2000, IHC 1:30-1:150 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human HNRNPH2 |
| Abbre | HNRNPH2 |
| Synonyms | FTP 3, FTP-3, FTP3, HNRH2, HNRNPH2, HNRPH', HNRPH2, Heterogeneous nuclear ribonucleoprotein H'', Heterogeneous nuclear ribonucleoprotein H2, heterogeneous nuclear ribonucleoprotein H2 (H'), hnRNP H'', hnRNP H2, hnRNPH' |
| Swissprot | |
| Calculated MW | 49 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus>nucleoplasm. |
| Concentration | 0.5 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs).The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA).These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport.While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm.The hnRNP proteins have distinct nucleic acid binding properties.The protein encoded by this gene has three repeats of quasi-RRM domains that binds to RNAs.It is very similar to the family member HNRPH1.This gene is thought to be involved in Fabray disease and X-linked agammaglobulinemia phenotype.Alternative splicing results in multiple transcript variants encoding the same protein.Read-through transcription between this locus and the ribosomal protein L36a gene has been observed. |
Other Clones
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Unconjugated
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