HMOX2 Polyclonal Antibody (E-AB-10367)
For research use only.
| Verified Samples |
Verified Samples in WB: Human liver, HepG2, Jurkat, K562 |
| Dilution | WB 1:200-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human , Mouse , Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant protein of human HMOX2 |
| Abbre | HMOX2 |
| Synonyms | HMOX 2, HMOX2, HMOX2 protein, HO 2, HO-2, HO2, Heme oxygenase (decycling) 2, Heme oxygenase (decyclizing) 2, Heme oxygenase 2, Hmox2, OTTHUMP00000159847 |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Microsome. Endoplasmic reticulum. |
| Concentration | 0.6 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cell Biology, Metabolism |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter.Heme oxygenase activity is induced by its substrate heme and by various nonheme substances.Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2.HMOX1 and HMOX2 belong to the heme oxygenase family.Alternative splice variants encoding the same protein have been identified at this locus. |
Other Clones
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Unconjugated
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