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Histone H3 Monoclonal Antibody (E-AB-22003)

AllSizePriceQty
200μL $ 399.00
120μL $ 240.00
60μL $ 143.00
20μL $ 73.00
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For research use only.

Verified Samples Verified Samples in WB: Hela, RAW264.7, Mouse brain, Rat brain
Verified Samples in IHC: Human uterus
Verified Samples in IF: Rat liver
Dilution WB 1:500-1:2000,  IHC 1:50-300,  IF 1:100-500,  IP 1:100-1:300
Isotype IgG
Host Mouse
Reactivity Human,  Mouse,  Rat,  Yeast
Applications WB,  IF
ClonalityMonoclonal
ImmunogenRecombinant Protein
AbbreHistone H3
SynonymsH3FA,  H3FB,  H3FC,  H3FD,  H3FF,  H3FH,  H3FI,  H3FJ,  H3FK,  H3FL,  HIST1H3A,  HIST1H3B,  HIST1H3C,  HIST1H3D,  HIST1H3E,  HIST1H3F,  HIST1H3G,  HIST1H3H,  HIST1H3I,  HIST1H3J,  Histone H3.1,  Histone H3/,  Histone H3/a,  Histone H3/b,  Histone H3/c,  Histone H3/d,  Histone H3/f
Swissprot
Calculated MW15 kDa
Observed MW 15 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular LocalizationExtracellular region or secreted, extracellular exosome, extracellular region, Nucleus, nuclear chromosome, nuclear chromosome, telomeric region, nuclear nucleosome, nucleoplasm, nucleus, Other locations: nucleosome, protein complex.
Concentration1 mg/mL
BufferPhosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Purification MethodProtein A purification
Research AreasCancer,  Epigenetics and Nuclear Signaling
Clone No.5B1
ConjugationUnconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
ShippingThe product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
backgroundCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Other Clones

1 Results

    Other Formats

    1 Results

    Unconjugated

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