Histone Deacetylase (HDAC) Activity Fluorometric Assay Kit (E-BC-F051)
For research use only.
| Detection Principle |
Histone deacetylases (HDAC) are a type of protease that play an important role in the structural modification of chromosomes and the regulation of gene expression. Earlier studies have shown that HDAC has a close relationship with tumors. The overexpression of HDAC can lead to various types of cancer as well as other neurological diseases, autoimmune diseases, inflammatory diseases, heart and lung diseases. Detection principle: HDAC specifically cleaves the fluorescent substrate, and the fluorescent group emits fluorescence which is detected at the excitation wavelength of 355 nm and the emission wavelength of 460 nm. Specific inhibitors are used in the kit to inhibit enzyme activity and eliminate the influence of non-specific enzyme activity. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=355 nm/460 nm) |
| Research Area | Metabolic Diseases |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.019 U/L |
| Detection Range | 0.019-1.027 U/L |
| Precision | inter-assay CV: 6.9-8.8% | intra-assay CV: 3.3-4.6% |
| Sample Volume | 40 μL |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Mouse muscle tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 293T cells | 1 |
| 1×10^6 Hela cells | 1 |
| Mouse serum | 1 |
| Human plasma | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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