High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents) (E-BC-K222-S)
For research use only.
| Detection Principle | The generated red purple pigment have a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and the HDL-C content in the sample can be calculated. |
| Synonyms | HDL-C |
| Sample Type | Serum,Plasma,Cell,Culture supernatant,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (600 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Metabolic Diseases |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Detection Range | 0.065-3.8 mmol/L |
| Sample Volume | 10 μL |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Porcine serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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