Hdroxyproline (HYP) Colorimetric Assay Kit (Alkali Hydrolysis Method) (E-BC-K061-S)
For research use only.
| Detection Principle | The oxidation product which produced by hydroxyproline under the action of oxidant react with dimethylaminobenzaldehyde and show a purplish red color. The content of hydroxyproline can be calculated by measuring the OD value at 550 nm. |
| Synonyms | Hyp |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (565 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.024 μg/mL |
| Detection Range | 0.024-5 μg/mL |
| Precision | inter-assay CV: 0.7-2.0% | intra-assay CV: 3.3-4.2% |
| Sample Volume | 1 mL |
| Assay Time | 2 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Fish scale | 4-10 |
| Fin tissue | 4-10 |
| Fishtail tissue | 4-10 |
| Mouse lung tissue | 1 |
| Mouse muscle tissue | 2-5 |
| Mouse leg bone tissue | 4-10 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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