For research use only. Order now, ship in 3 days
| Product Introduction | Ham's F-12 Nutrient Mixture was designed by Ham in 1969 based on Ham's F-10 nutrient mixture, which was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as the basic culture medium for serum free culture. At low serum levels, it is especially suitable for single cell culture and clonal culture. After adding serum, it is also widely used in the culture of cancer cells and primary cells, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, and chicken embryonic cells. In addition, the DMEM/F12 medium obtained by mixing Ham's F-12 in equal volume with DMEM is more nutritious and widely used. |
| Concentration | 1× |
| Format | Liquid |
| Mycoplasmal Detection | Negative |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Endotoxin Level | ＜3 |
| Storage | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping | Room Temperature |
| Expiration Date | 24 months |
| Matters Need Attention | 1. This product is for research use only. 2. This product is sterilized by 0.1 μm filtration. 3. It is necessary to pay attention to the aseptic operation and avoid the contamination. |
Other Clones
1 Results
Other Formats
1 Results
Q1:Will improper storage of my culture reagents affect the growth rate of my culture?
Yes. Store animal sera at -5 to -20℃. Store media at 2 to 8℃; use within recommended shelf life period. Store complete media (supplemented) at 2 to 8℃, and for complete medium the recommended shelf life is 3 to 6 months. Additionally, minimize exposure of sera and media to light.
Q2:Where can I find the osmolality for Medium?
The osmolality is listed in the COA for the particular lot number of the medium.
Q3:My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Q4:I see a precipitate in my medium, and a change in the pH. What should I do?
This is most likely a sign of bacterial or fungal contamination. We would recommend discarding the media, and trying to decontaminate the culture.
Q5:How long can I keep my media after supplementing with serum?
Generally speaking, media can be used for up to 3-6 months after supplementation with serum， at 2 to 8℃ low temperature environment preservation.
Q6:How light sensitive is media? Should I also be protecting it from LED light?
While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.When placed in a light-proof 4°C refrigerator with the door closed, the culture medium will be kept away from light.
Q7:Do you provide customized culture media services?
Yes. We can customize culture media with reduced components or changes in the concentration of existing components.
