HA-Tag Monoclonal Antibody (E-AB-48021)
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For research use only.
| Verified Samples |
Verified Samples in WB: HA-Tag fused recombinant protein Verified Samples in IF: 293F Verified Samples in IP: 293F Verified Samples in FCM: CHO |
| Dilution | WB 1:5000-10000, IP 3μg/sample, IF 1:400-800, FCM 0.2μg |
| Isotype | IgG2a |
| Host | Mouse |
| Reactivity | All |
| Applications | WB, IF, IP, FCM |
| Clonality | Monoclonal |
| Immunogen | Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin. |
| Abbre | HA-Tag |
| Synonyms | HA1 , Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, hemagglutinin, HA epitope tag, HA2 |
| Calculated MW | 28 kDa |
| Observed MW |
28 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS, pH 7.2. Contains 0.05% proclin 300. |
| Purification Method | Protein A/G Purification |
| Clone No. | 5F7 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. |
Other Clones
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Other Formats
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Unconjugated
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