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GSPT2 Polyclonal Antibody (E-AB-52994)

AllSizePriceQty
200μL $ 399.00
120μL $ 240.00
60μL $ 143.00
20μL $ 73.00
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For research use only.

Verified Samples Verified Samples in WB: 231
Verified Samples in IHC: Human cervical cancer
Dilution WB 1:500-1:2000,  IHC 1:30-1:150
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse
Applications WB,  IHC
ClonalityPolyclonal
ImmunogenFusion protein of human GSPT2
AbbreGSPT2
SynonymsERF3B,  Eukaryotic peptide chain release factor GTP-binding subunit ERF3B,  Eukaryotic peptide chain release factor subunit 3b,  G1 to S phase transition 2,  G1 to S phase transition protein 2 homolog,  GST2 ,  Gspt2,  eRF3b,  peptide chain release factor 3
Swissprot
Calculated MW69 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular LocalizationCytoplasm.
Concentration0.84 mg/mL
BufferPhosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification MethodAntigen affinity purification
Research AreasEpigenetics and Nuclear Signaling
ConjugationUnconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
ShippingThe product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
backgroundThis gene encodes a GTPase that belongs to the GTP-binding elongation factor family. The encoded protein is a polypeptide release factor that complexes with eukaryotic peptide chain release factor 1 to mediate translation termination. This protein may also be involved in mRNA stability.
Other Clones

1 Results

    Other Formats

    1 Results

    Unconjugated