GPR6 Polyclonal Antibody (E-AB-19291)
For research use only.
| Verified Samples |
Verified Samples in WB: Human placenta Verified Samples in IHC: Human colorectal cancer |
| Dilution | WB 1:1000-1:5000, IHC 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human GPR6 |
| Abbre | GPR6 |
| Synonyms | G protein coupled receptor 6, G-protein coupled receptor 6, GPR 6, GPR6, Sphingosine 1 phosphate receptor GPR 6, Sphingosine 1 phosphate receptor GPR6, Sphingosine 1-phosphate receptor GPR6 |
| Swissprot | |
| Calculated MW | 38 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane. Detected in the intracellular compartments. It is currently unclear whether this is a cell surface or intracellular receptor. |
| Concentration | 1.2 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Neuroscience, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | G protein-coupled receptor 6, also known as GPR6, is a protein which in humans is encoded by the GPR6gene.GPR6 is a member of the G protein-coupled receptor family of transmembrane receptors.It has been reported that GPR6 is both constitutively active but in addition is further activated by sphingosine-1-phosphate.GPR6 up-regulates cyclic AMP levels and promotes neurite outgrowth. |
Other Clones
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Unconjugated
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