GOLM1/GP73 Monoclonal Antibody (AN200222P)
For research use only.
| Verified Samples |
Verified Samples in WB:?293T Verified Samples in IF: Hela |
| Dilution | WB 1:500-1:1000, ICC/IF 1:100-1:500 |
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB, ICC/IF |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human GOLM1/GP73 Protein |
| Abbre | GOLM1 |
| Synonyms | HEL, UNQ, PRO, GOLM, Golgi Phosphoprotein, Golgi Membrane Protein, Golgi Membrane Protein GP, C9orf, GOLPH, bA379P, GOLM1, C9orf155, GOLPH2, GP73, HEL46, PSEC0257, bA379P1.3, Golgi Membrane Protein 1, Golgi Membrane Protein GP73, Golgi Phosphoprotein 2, PSEC0242, UNQ686, PRO1326 |
| Swissprot | |
| Calculated MW | 45 kDa |
| Observed MW |
75 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocytes-derived syncytial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction |
| Clone No. | 4B7 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene. |
Other Clones
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Unconjugated
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