Glycosylated Serum Protein (GSP) Colorimetric Assay Kit (E-BC-K069-M)
For research use only.
| Detection Principle | Glycosylated serum protein (GSP), also called serum fructosamine, is formed by non-enzymatic reaction between glucose and serum protein in blood, and its content can effectively reflect the average blood glucose level in 1-3 weeks before measurement. Glycosylated serum protein is not affected by the temporary fluctuation of blood glucose concentration and provides a good indicator for the study of diabetes and long-term blood glucose level control. GSP with ketoamine structure is able to react with tetrazole blue under alkaline conditions to form purplish red product, which has a characteristic absorption peak at 530 nm, the concentration of GSP can be calculated by measuring the OD value. |
| Synonyms | GSP |
| Sample Type | Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (520-550 nm, optimum wavelength: 530 nm) |
| Research Area | Amino Acids And Proteins , Metabolic Diseases |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.06 mmol/L |
| Detection Range | 0.06-4.0 mmol/L |
| Precision | inter-assay CV: 6.3% | intra-assay CV: 4.4% |
| Sample Volume | 10 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| Mouse serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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