Glycolysis Stress Fluorometric Assay Kit (E-BC-F084)
For research use only.
| Detection Principle |
Glycolysis is an important ATP generation pathway in eukaryotic cells. Glycolytic stress testing can directly measure the extracellular acidification rate (ECAR) to evaluate the changes of glycolytic process in living cells, and can obtain key parameters for a comprehensive assessment of glycolytic pathway, including: glycolysis, glycolytic capacity, glycolytic reserve and non-glycolytic acidification. This kit uses 2-deoxy-D-glucose (2-DG) and oligomycin to facilitate in-depth analysis of cellular glycolytic flux: 2-DG quantifies nonglycolytic extracellular acidification (ECA) by blocking glycolysis through competitive hexokinase inhibition; Oligomycin inhibits ATP synthetase and therefore prevents aerobic ATP production, forcing the cell to increase glycolytic flux to meet ATP demand. Such compensatory glycolysis levels achievable under the test conditions could reveal glycolytic perturbations that are not apparent under basal conditions and help assess glycolytic capacity with limited ATP demand. |
| Sample Type | Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=490 nm/535 nm) |
| Research Area | Energy Metabolism |
| Other Reagents Required | DMSO |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Assay Time | 2 h |
Other Clones
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® Violet 450
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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