Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit (E-BC-K790-M)
For research use only.
| Detection Principle |
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), a key enzyme in the glycolytic pathway, catalyzes the oxidation of glyceraldehyde-3-phosphate to 1, 3-diphosphoglycerate. This response is closely related to the gluconeogenic pathway, the maintenance of blood glucose concentrations in the body, and the development of diabetes. GAPDH gene is a housekeeping gene, which is expressed at a high level in almost all tissues. Therefore, GAPDH widely exists in tissues and cells and plays an important role in the disorders of glucose, lipid and protein metabolism in the body. This kit can calculate the enzyme activity of GAPDH by measuring the OD value change at 450 nm per unit time of the product produced by GAPDH catalyzing the oxidation of glyceraldehyde-3-phosphate. |
| Synonyms | GAPDH |
| Sample Type | Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (430-470 nm, optimum wavelength: 450 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.49 U/L |
| Detection Range | 0.49-50.00 U/L |
| Precision | inter-assay CV: 3.2-8.6% | intra-assay CV: 3.2-5.0% |
| Sample Volume | 40 μL(tissue/cell homogenate) |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenization | 1 |
| 10% Mouse kidney tissue homogenization | 1 |
| 10% Mouse lung tissue homogenization | 1 |
| 10% Spinach tissue homogenization | 1 |
| 10% Squash tissue homogenization | 1 |
| 10% Cabbage tissue homogenization | 1 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 CHO cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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