Glutathione-S-Transferase (GST) Activity Assay Kit(DTNB method) (E-BC-K800-M)
For research use only.
| Detection Principle | GST can catalyze the binding of reduced glutathione (GSH) to dinitrobenzene (CDNB). The enzyme activity is indicated by measuring the substrate GSH binding rate with dinitrodiphenyl in unit time, the reaction of the rest of the GSH acts with disulfide double nitro benzoic acid (DTNB) to form yellow glucosinolates nitro benzoic acid anion (TNB), the concentration of which is determined to calculate the reduction of GSH. Thus, the activity of glutathione S-transferase (GST) was calculated indirectly by measuring the OD value at 412 nm. |
| Synonyms | GST |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-420 nm, optimum wavelength: 412 nm) |
| Research Area | Oxidative Stress , Liver And Renal Function |
| Other Reagents Required | PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 2.1 U/L |
| Detection Range | 2.1-92.8 U/L |
| Precision | inter-assay CV: 6.4% | intra-assay CV: 1.8% |
| Sample Volume | 40 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human plasma (serum) | 1 |
| Horse serum | 1 |
| Rat serum | 1 |
| Rabbit serum | 1 |
| Porcine serum | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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