γ-Glutamyl Transferase (GGT/γ-GT) Activity Assay Kit (E-BC-K126-M)
For research use only.
| Detection Principle | γ-GT catalyzes the transfer of gamma glutamyl group from glutamyl p-nitroaniline to N-glycyl glycine to produce p-nitroaniline, which has characteristic absorption peak at 405 nm. The activity of γ-GT can be calculated according to the changing rate of absorbance value. |
| Synonyms | GGT;γ-GT |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (405 nm) |
| Research Area | Liver And Renal Function |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.88 U/L |
| Detection Range | 0.88-399.4 U/L |
| Precision | inter-assay CV: 6.2% | intra-assay CV: 4.2% |
| Sample Volume | 25 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Mouse serum | 1 |
| Human serum | 1 |
| Rat serum | 1 |
| Dog serum | 1 |
| Human plasma | 1 |
| Horse serum | 1 |
| Porcine serum | 1 |
| Human hydrothorax | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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