Glutamine Synthetase (GS) Activity Colorimetric Assay Kit (E-BC-K664-M)
For research use only.
| Detection Principle | Glutamine synthetase (GS) is one of the central enzymes in nitrogen metabolism With ATP consumption, GS catalyzes glutamate to produce glutamine. The latter is an amino donor of various nitrogenous substances such as proteins, nucleic acids and various coenzymes. As a conditionally essential amino acid for the human body, the production of glutamine has received extensive attention in recent years. GS catalyzes ammonium ion and glutamic acid to synthesize amide, and produces chromogenic substance under the action of invertase and chromogenic agent. The maximum absorption peak is at 570 nm. The activity of GS in samples is calculated by measuring OD value at 570 nm and standard curve. |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (560-580 nm, optimum wavelength: 570 nm) |
| Research Area | Liver And Renal Function , Tricarboxylic Acid（TCA）Cycle |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.25 U/L |
| Detection Range | 0.25-25 U/L |
| Precision | inter-assay CV: 5.3-6.8% | intra-assay CV: 2.0-4.0% |
| Sample Volume | 40 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat kidney tissue homogenate | 4-10 |
| 10% Rat liver tissue homogenate | 5-20 |
| 10% Rat spleen tissue homogenate | 2-10 |
| 10% Rat heart tissue homogenate | 2-10 |
| 10% Rat lung tissue homogenate | 2-10 |
| 10% Rat brain tissue homogenate | 5-20 |
| 10% Mouse liver tissue homogenate | 5-20 |
| 10% Mouse brain tissue homogenate | 2-10 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Human serum | 1 |
| Porcine serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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