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For research use only.
| Detection Principle | GOGAT catalysis the reaction that transfer the amino from glutamine to a-KG to form two molecules of glutamic acid using NADH as the electron donor. The decreasing rate of NADH that can be measured at 340 nm can reflect the activity of GOGAT. |
| Synonyms | GOGAT |
| Sample Type | Serum,plant,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (330-350 nm, optimum wavelength: 340 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 8.84 U/L |
| Detection Range | 8.84-321.05 U/L |
| Precision | inter-assay CV: 5.8% | intra-assay CV: 2.5% |
| Sample Volume | 20 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat kidney tissue homogenate | 2-4 |
| 10% Rat liver tissue homogenate | 2-4 |
| 10% Rat heart tissue homogenate | 2-4 |
| 10% Mouse liver tissue homogenate | 2-4 |
| Bovine serum | 1 |
| 10% pleurotus cornucopiae tissue homogenate | 1 |
| 10% beech mushroom tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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