Galectin 3 Monoclonal Antibody (E-AB-22006)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela Verified Samples in IHC: Human thyroid Verified Samples in IF: Human lung cancer |
| Dilution | WB 1:1000-1:3000, IHC 1:100-1:300, IF 1:100-200 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB, IHC-p, IF |
| Clonality | Monoclonal |
| Immunogen | Synthetic Peptide |
| Abbre | Galectin-3 |
| Synonyms | 35 kDa lectin, CBP 35, CBP35, Carbohydrate binding protein 35, Carbohydrate-binding protein 35, GAL3, GALBP, GALIG, GBP, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, Galectin 3 internal gene, Galectin-3, Galectin3, IgE binding protein, IgE-bi, included |
| Swissprot | |
| Calculated MW | 26 kDa |
| Observed MW |
26 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. |
| Tissue Specificity | A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Protein A purification |
| Research Areas | Cancer, Cell Biology, Neuroscience |
| Clone No. | 1A2 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. |
Other Clones
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Other Formats
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Unconjugated
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