Fluoride Resistant Acid Phosphatase (FRAP) Activity Assay Kit (E-BC-K872-M)
For research use only.
| Detection Principle | Fluoride resistant acid phosphatase (FRAP) is not inhibited by fluoride ions. It catalyzes the substrate to produce p-nitrophenol, which has a maximum absorption peak at 405 nm. The activity of FRAP can be calculated by measuring the OD value at 405 nm. |
| Synonyms | FRAP |
| Sample Type | Serum,Plasma,Cell,Animal tissue sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (390-415 nm, optimum wavelength: 405 nm) |
| Research Area | Metabolic Diseases |
| Other Reagents Required | Double distilled water, Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.61 U/L |
| Detection Range | 0.61-49.37 U/L |
| Precision | inter-assay CV: 0.08 | intra-assay CV: 3.6% |
| Sample Volume | 40 μL |
| Assay Time | 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 3-7 |
| 10% Mouse kidney tissue homogenate | 3-7 |
| 10% Mouse heart tissue homogenate | 3-7 |
| 10% Mouse lung tissue homogenate | 3-7 |
| Rat plasma | 5-7 |
| Human serum | 1-3 |
| Bovine serum | 1-3 |
| 1 × 10^6 HL-60 cell | 1 |
| 1 × 10^6 293T cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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