Flag-Tag Polyclonal Antibody (E-AB-20045)
For research use only.
| Verified Samples |
Verified Samples in WB: Recombinant protein |
| Dilution | WB 1:5000-20000 |
| Isotype | IgG |
| Host | Rabbit |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Synthetic Peptide |
| Abbre | Flag-Tag |
| Synonyms | DDDDK epitope tag, DYKDDDDK epitope tag |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Tags and Cell Markers |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression.The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors.This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag.The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature.As a result, the FLAG peptide is more likely to be accessible to antibodies. |
Other Clones
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Other Formats
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Unconjugated
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