FCGR3A Polyclonal Antibody (E-AB-70305)
For research use only.
| Verified Samples |
Verified Samples in WB: THP-1, Jurkat, RAW264.7, Mouse spleen, Mouse lung, Mouse thymus, Rat spleen, Rat lung, Rat thymus |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Human CD16A |
| Abbre | FCGR3A |
| Synonyms | CD 16, CD 16a, CD16, CD16B, CD16a, CD16a antigen, CD16b antigen, Fc fragment of IgG, Fc fragment of IgG low affinity IIIa receptor, Fc fragment of IgG low affinity IIIa receptor (CD16), Fc fragment of IgG receptor IIIa, low affinity III, receptor ( |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW |
55 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane. Secreted. Exists also as a soluble receptor. |
| Concentration | 420 μg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Immunology, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses.This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1.The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. |
Other Clones
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Unconjugated
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