ERK2/MAPK1/MAPK2 Monoclonal Antibody (AN200155P)
For research use only.
| Verified Samples | Verified Samples in WB:?A431, HepG2, A549, Jurkat, Hela, ERK2 konckout Hela |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human ERK2/MAPK1/MAPK2 Protein |
| Abbre | MAPK1 |
| Synonyms | ERK, MAPK1/MAPK, MAPK, MAPK1/MAPK3, ERK2, PRKM1, PRKM2, MAPK1, ERK 2, ERK-2, ERT1, Extracellular Signal Regulated Kinase 2, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK2, mitogen-activated, mitogen-activated protein kinase 1, Mitogen-activated protein kinase 2, MK01, p38, p40, p41, p41mapk, P42MAPK, p42-MAPK, protein kinase, protein tyrosine kinase ERK2 |
| Swissprot | |
| Calculated MW | 40 kDa |
| Observed MW |
40 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Signal Transduction, Stem Cells, Cancer |
| Clone No. | 2B10 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. |
Other Clones
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Unconjugated
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