EPHA3/EPHA4/EPHA5 Polyclonal Antibody (E-AB-92205)
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For research use only.
| Verified Samples |
Verified Samples in IHC: Rat kidney, Human placenta Verified Samples in IF: C6, NIH/3T3 |
| Dilution | IHC 1:50-1:200, IF 1:50-1:200 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF |
| Clonality | Polyclonal |
| Immunogen | A synthetic peptide of human EPHA3/EPHA4/EPHA5 |
| Abbre | EPHA3/EPHA4/EPHA5 |
| Synonyms | AW492086,Cek4,EC 2.7.10.1,EK4,End3,Eph receptor A3,EPH-like kinase 4,EPH-like tyrosine kinase 1,EPHA3,EPHA3,Ephrin receptor EphA3,Ephrin type-A receptor 3,ETK 1,ETK,ETK1,HEK 4,HEK,HEK4,Human embryo kinase 1,Human embryo kinase,Mek4,MGC109882,Receptor tyro |
| Swissprot | |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Actin cytoskeleton, cytosol, early endosome, extracellular region, nuclear membrane, nucleoplasm, plasma membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Affinity purification |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development. |
Other Clones
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Other Formats
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Unconjugated
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