EpCAM/TROP-1 Polyclonal Antibody(Capture/Detector) (AN004000P)
For research use only.
| Verified Samples |
Verified Samples in ELISA: Recombinant Mouse EpCAM/TROP-1 protein, Mouse serum, Mouse plasma, Human serum, Human plasma Verified Samples in WB: Mouse colon |
| Dilution | ELISA Capture 2-8 μg/mL, ELISA Detector 0.1-0.4 μg/mL, WB 1:1000-1:2000 |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Mouse, Human |
| Applications | ELISA Capture/Detector, WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Mouse EpCAM/TROP-1 Protein expressed by Mammalian |
| Abbre | EpCAM/TROP-1 |
| Synonyms | EGP, Megp, Tacstd, EGP314, Ep-CAM, Megp314, Tacstd1, Epcam, MK-1, KSA, EGP40, 17 1A, 323/A3, 35-KD glycoprotein, Adenocarcinoma associated antigen, Adenocarcinoma-associated antigen, Antigen identified by monoclonal AUA1, AUA1, CD326, CD326 antigen, Cell surface glycoprotein Trop 1, Cell surface glycoprotein Trop 2, Cell surface glycoprotein Trop-1, chromosome 4, CO 17A, CO17 1A, CO17A, DIAR5, EGP 2, EGP2, EGP-2, EGP40, Ep CAM, EpCAM1, epithelial cell adhesion molecule, Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD), Epithelial cell surface antigen, Epithelial cellular adhesion molecule, Epithelial glycoprotein, Epithelial glycoprotein 1, Epithelial glycoprotein 314, ESA, GA733 1, GA733 2, GA733-2, gastrointestinal tumor-associated antigen 2, gp4, gp40, hEGP 2, hEGP314, HNPCC8, Human epithelial glycoprotein 2, KS 1/4 antigen, KS1/4, KSA, Ly74, Lymphocyte antigen 74, M1S 1, M1S2, M4S1, Major gastrointestinal tumor associated protein GA733 2, Major gastrointestinal tumor-associated protein GA733-2, Membrane component, Membrane component chromosome 4 surface marker (35kD glycoprotein), MIC18, MK 1, MK-1, Neurotrophic tyrosine kinase receptor-related 1, Protein 289A, receptor tyrosine kinase-like orphan receptor 1, ROR1, surface marker, surface marker 1, TACD1, Tacsd1, TROP1, Tumor associated calcium signal transducer 1, Tumor associated calcium signal transducer 2 precursor, Tumor-associated calcium signal transducer 1, tyrosine-protein kinase transmembrane receptor ROR1 |
| Swissprot | |
| Calculated MW | 35 kDa |
| Observed MW |
40-45 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300. |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type I transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway. |
Other Clones
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Unconjugated
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