ENO2 Polyclonal Antibody (D-AB-10203L)
For research use only.
| Verified Samples |
Verified Samples in WB: SH-SY5Y, Hela, Mouse brain, Rat brain |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human ENO2 protein expressed by E.coli |
| Abbre | ENO2 |
| Synonyms | 2-phospho-D-glycerate hydro-lyase, ENO2, Enolase 2, Gamma-enolase, Neural enolase, Neuron-specific enolase (NSE) |
| Swissprot | |
| Calculated MW | 47 kDa |
| Observed MW |
47 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer, Metabolism, Developmental Biology, Neuroscience, Stem Cells, Tags and Cell Markers |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Enolase 2(2-phospho-D-glycerate hydrolyase; also Neuron-specific Enolase,NSE,neural enolase and gamma-enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia,is known to catalyze the generation of phosphoenolpyruvate,and is suggested to possess neurotrophic activity for neurons,likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids(aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer,or heterodimer with alpha-enolase. Full-length human Enolase 2 shares 99% aa identity with both mouse and canine Enolase 2. It shares 83% aa identity with human enolases 1 and 3. |
Other Clones
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Other Formats
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Unconjugated
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