ENO2 Monoclonal Antibody (AN200228P)
For research use only.
| Verified Samples |
Verified Samples in WB:?HepG2, SH-SY5Y, Hela, Jurkat Verified Samples in IHC: Human pancreas, Human brain |
| Dilution | WB 1:500-1:2000, IHC-P 1:100-1:500 |
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB, IHC-P |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human ENO2 Protein |
| Abbre | ENO2 |
| Synonyms | ENO, HEL-S, enolase, ENO2, HEL-S-279, NSE, enolase 2, 2-phospho-D-glycerate hydro-lyase, Enolase γ, Gamma-enolase, ENOG, Neural enolase, Neuron-specific enolase, 2 phospho D glycerate hydrolyase, Eno 2, Enolase 2 (gamma, Enolase 2 gamma neuronal, Enolase2, Epididymis secretory protein Li 279, Gamma enolase, HEL S 279, Neuron specific enolase, Neuron specific gamma enolase, neuronal enriched enolase, neuronal), Neurone specific enolase, Neuron-specific enolase (NSE) |
| Swissprot | |
| Calculated MW | 47 kDa |
| Observed MW |
50 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Cell membrane |
| Tissue Specificity | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Tags & Cell Markers, Stem Cells, Cancer, Developmental Biology, Metabolism, Signal Transduction, Epigenetics and Nuclear Signaling |
| Clone No. | 5B5 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. |
Other Clones
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Unconjugated
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