Enhanced Cell Malondialdehyde (MDA) Colorimetric Assay Kit (E-BC-K814-M)
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For research use only.
| Detection Principle | Malondialdehyde (MDA) in the degradation products of peroxidized lipids can react with thiobarbituric acid (TBA) under high temperature and acidic conditions to produce the red-brown product 3,5,5 '-trimethyloxazole-2, 4-dione (trimethine), and this substance has the maximum absorption peak at 532 nm. |
| Sample Type | Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-540 nm, optimum wavelength: 532 nm) |
| Research Area | Oxidative Stress |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.19 nmol/mL |
| Detection Range | 0.19-100.00 nmol/mL |
| Precision | inter-assay CV: 2.3-3.5% | intra-assay CV: 2.1-3.3% |
| Sample Volume | 0.1 mL (cell homogenate) |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 2×10^6 A549 cells | 1 |
| 2×10^6 HepG2 cells | 1 |
| 2×10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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