EEF1D Polyclonal Antibody (E-AB-19083)
For research use only.
| Verified Samples |
Verified Samples in WB: A549, A172, HepG2 Verified Samples in IHC: Human thyroid cancer, Human ovarian cancer |
| Dilution | WB 1:1000-1:5000, IHC 1:50-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human EEF1D |
| Abbre | EEF1D |
| Synonyms | Antigen NY CO 4, Antigen NY-CO-4, EEF 1D, EEF1D, EF 1 delta, EF 1D, EF-1-delta, EF1D, Elongation factor 1 delta, Elongation factor 1-delta, Eukaryotic translation elongation, eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) |
| Swissprot | |
| Calculated MW | 31 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus. |
| Concentration | 1.14 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Epigenetics and Nuclear Signaling, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | EEF1D,also named as EF1D and EF 1 delta,belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex,which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage,probably by ATM or ATR. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human EEF1D. |
Other Clones
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Unconjugated
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